inverted widefield microscope eclipse te2000-u Search Results


widefield microscope  (Nikon)
99
Nikon widefield microscope
Widefield Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+widefield+microscope+eclipse+te2000-u/Objectives/pmc02843500-170-18-20
Average 99 stars, based on 1 article reviews
widefield microscope - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
xcite  (Excelitas corp)
99
Excelitas corp xcite
Xcite, supplied by Excelitas corp, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+widefield+microscope+eclipse+te2000-u/xcite/custom%40xcite%4028928238
Average 99 stars, based on 1 article reviews
xcite - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
widefield microscope te2000-u  (Omicron Laserage Laserprodukte GmbH)
90
Omicron Laserage Laserprodukte GmbH widefield microscope te2000-u
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
Widefield Microscope Te2000 U, supplied by Omicron Laserage Laserprodukte GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+widefield+microscope+eclipse+te2000-u/widefield+microscope+te2000+u/pmc03105540-170-11-29
Average 90 stars, based on 1 article reviews
widefield microscope te2000-u - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
1394 orca-era camera  (Hamamatsu)
90
Hamamatsu 1394 orca-era camera
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
1394 Orca Era Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+widefield+microscope+eclipse+te2000-u/1394+orca+era+camera/pmc04661044-30-25-28
Average 90 stars, based on 1 article reviews
1394 orca-era camera - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
orca er camera  (Hamamatsu)
90
Hamamatsu orca er camera
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
Orca Er Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+widefield+microscope+eclipse+te2000-u/orca+er+camera/pmc04539978-232-30-33
Average 90 stars, based on 1 article reviews
orca er camera - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
orcaflash4.0 lt camera  (Hamamatsu)
90
Hamamatsu orcaflash4.0 lt camera
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
Orcaflash4.0 Lt Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+widefield+microscope+eclipse+te2000-u/orca+flash+4+0+camera/pm38137015-116-26-25
Average 90 stars, based on 1 article reviews
orcaflash4.0 lt camera - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
d-104 microscope photometer  (Photon Technologies Inc)
90
Photon Technologies Inc d-104 microscope photometer
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
D 104 Microscope Photometer, supplied by Photon Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+widefield+microscope+eclipse+te2000-u/d+104+microscope+photometer/pmc05672804-381-59-62
Average 90 stars, based on 1 article reviews
d-104 microscope photometer - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
electron-multiplied charge coupled device (emccd) camera (rolera em-c 2  (QImaging)
90
QImaging electron-multiplied charge coupled device (emccd) camera (rolera em-c 2
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
Electron Multiplied Charge Coupled Device (Emccd) Camera (Rolera Em C 2, supplied by QImaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+widefield+microscope+eclipse+te2000-u/rolera+mgi+plus+back+illuminated+emccd+camera/pmc03758648-71-55-65
Average 90 stars, based on 1 article reviews
electron-multiplied charge coupled device (emccd) camera (rolera em-c 2 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
led p e exciation system  (CoolLED Inc)
90
CoolLED Inc led p e exciation system
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
Led P E Exciation System, supplied by CoolLED Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+widefield+microscope+eclipse+te2000-u/led+p+e+exciation+system/bio_rxiv__2024__07__18__604162-122-18-23
Average 90 stars, based on 1 article reviews
led p e exciation system - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
ds qi2 camera  (Nikon)
99
Nikon ds qi2 camera
WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved <t>widefield</t> images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.
Ds Qi2 Camera, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+widefield+microscope+eclipse+te2000-u/NIS-Elements/bio_rxiv__2024__07__18__604162-122-27-29
Average 99 stars, based on 1 article reviews
ds qi2 camera - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier

Image Search Results


WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved widefield images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.

Journal: The Journal of Cell Biology

Article Title: Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis

doi: 10.1083/jcb.201009119

Figure Lengend Snippet: WASH localizes to postlysosomes and is required for postlysosomal actin coats. (a) Domain architecture of WASP subfamilies. (b) Deconvolved widefield images of parent (AX2) and WASH-null ( wshA − ) cell lines fixed and stained with phalloidin. WASH-null cells lack large F-actin–coated vesicles (arrows in AX2). (c) Loss of actin-coated vesicles. Parental ( n = 82) and wshA − ( n = 79) cells were fixed and stained with phalloidin. Vesicles were counted in all planes of focus; macropinosomes were excluded. Error bars represent SEM. (d) Expression of GFP-WASH (middle panel and green) in fixed, phalloidin-stained (left panel and red) wshA − cells. (e) Expression of GFP-WASHΔVCA (green) in fixed, phalloidin-stained (left panel and red) wshA − cells. Bars, 10 µm.

Article Snippet: For qualitative high speed acquisition, cells were imaged on a TE2000-U widefield microscope with a 100× 1.49 NA objective, with illumination provided by 473- and 561-nm solid-state lasers (Deepstar; Omicron Laserage Laserprodukte GmbH).

Techniques: Staining, Expressing

WASH causes recycling of the V-ATPase. (a) Confocal imaging of wshA − cells expressing GFP-WASH and VatB-mRFP after endocytosis of 0.5-µm agarose beads. Frames are taken from Video 3 . Insets show a magnified view of the vesicle on which WASH is acting. (b) Quantification of various vesicular proteins during postlysosome formation. Graphs show that the loss of V-ATPase immediately follows the arrival of WASH and coronin, whereas the previously described postlysosome marker vacuolin is present much earlier than neutralization and rises steadily. The representative curves are from an experiment that was performed at least five times. (c) Rapid widefield oblique illumination imaging of small vesicles containing both GFP-WASH and VatB-mRFP budding a single lysosome. Frames are taken from Video 4 . Bar, 1 µm.

Journal: The Journal of Cell Biology

Article Title: Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis

doi: 10.1083/jcb.201009119

Figure Lengend Snippet: WASH causes recycling of the V-ATPase. (a) Confocal imaging of wshA − cells expressing GFP-WASH and VatB-mRFP after endocytosis of 0.5-µm agarose beads. Frames are taken from Video 3 . Insets show a magnified view of the vesicle on which WASH is acting. (b) Quantification of various vesicular proteins during postlysosome formation. Graphs show that the loss of V-ATPase immediately follows the arrival of WASH and coronin, whereas the previously described postlysosome marker vacuolin is present much earlier than neutralization and rises steadily. The representative curves are from an experiment that was performed at least five times. (c) Rapid widefield oblique illumination imaging of small vesicles containing both GFP-WASH and VatB-mRFP budding a single lysosome. Frames are taken from Video 4 . Bar, 1 µm.

Article Snippet: For qualitative high speed acquisition, cells were imaged on a TE2000-U widefield microscope with a 100× 1.49 NA objective, with illumination provided by 473- and 561-nm solid-state lasers (Deepstar; Omicron Laserage Laserprodukte GmbH).

Techniques: Imaging, Expressing, Marker, Neutralization